目的:探究微小RNA-141(miR-141)在膀胱癌组织中的表达及与肿瘤分级的相关性。方法:选取2012年3月至2014年3月山西医科大学第二医院泌尿外科收治的80例膀胱癌患者，男67例，女13例，年龄(68.23±3.57)岁，年龄范围为61～83岁。采用实时荧光定量逆转录聚合酶链反应(PCR)检测膀胱癌组织及癌旁正常组织中miR-141 mRNA相对表达量，分析miR-141表达与膀胱癌患者临床病理特征的相关性，分析miR-141表达与膀胱癌患者预后的相关性，比较miR-141低表达患者与miR-141高表达患者总生存率与无进展生存率。结果:MiR-141 mRNA在膀胱癌组织相对表达量(4.93±0.88)显著高于癌旁正常组织(0.83±0.11)，差异有统计学意义(n P0.05)；肿瘤分级、肿瘤分期、肌层浸润、肿瘤转移不同的膀胱癌患者癌组织中miR-141 mRNA相对表达量比较，差异有统计学意义(n P<0.05)；肿瘤分级、肿瘤分期、肿瘤转移、miR-141表达不同的膀胱癌患者3年生存率比较，差异有统计学意义(n P<0.05)；肿瘤分级、肿瘤分期、肿瘤转移、miR-141表达均是影响膀胱癌患者预后的独立危险因素(n P<0.05)；miR-141低表达患者总生存率[71.0%(22/31)]、无进展生存率[64.5%(20/31)]显著高于miR-141高表达患者[20.4%(10/49)、24.5%(12/49)]，差异有统计学意义(n P<0.05)。n 结论:miR-141在膀胱癌组织中呈高表达，miR-141表达与膀胱癌患者肿瘤分级、肿瘤分期、肿瘤转移及预后呈相关性，这可为膀胱癌患者早期诊断及预后评估提供参考依据。“,”Objective:To investigate the expression of microRNA-141(miR-141) in bladder cancer and its correlation with tumor grade.Methods:A total of 80 patients with bladder cancer admitted to the department of Urology of the Second Hospital of Shanxi Medical University from March 2012 to March 2014 were selected, including 67 males and 13 females, aged(68.23±3.57)years old, ranging from 61 to 83 years old.The relative expression of miR-141 mRNA in bladder cancer tissues and adjacent normal tissues were detected by real-time polymerase chain reaction(RT-PCR). Correlation of miR-141 expression with clinicopathological characteristics and prognosis of bladder cancer patients was analyzed.The overall survival rate(OS)and progression-free survival rate(PFS)were compared between patients with low miR-141 expression and high miR-141 expression.Results:The relative expression of miR-141 mRNA in bladder cancer tissues was significantly higher than that in normal adjacent tissues [(4.93±0.88)versus(0.83±0.11), n P0.05). The relative expression of miR-141 mRNA had significant difference among bladder cancer patients with different tumor grades, tumor stages, muscular invasion and tumor metastasis(n P<0.05). The 3-year survival rate of bladder cancer patients with different tumor grades, tumor stages, tumor metastasis, and miR-141 expression showed significant difference(n P<0.05). Tumor grade, tumor stage, tumor metastasis, and miR-141 expression were independent risk factors that affect the prognosis of bladder cancer patients(n P<0.05). OS and PFS of miR-141 low expression patients were significantly higher than those of miR-141 high expression patients, with statistic difference[70.97%(22/31)versus 20.41%(10/49); 64.52%(20/31)versus 24.49%(12/49),n P<0.05].n Conclusion:The miR-141 is highly expressed in bladder cancer patients, and is closely related to tumor grade, tumor stage, tumor metastasis and prognosis of bladder cancer patients, which can provide a reference for early diagnosis and prognosis of bladder cancer patients.