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目的:探讨在急性髓性白血病(AML)中,CD40、CD40L、OX40抗原的表达与白血病相关基因Bcl-2和Bcl-xL表达的相关性。方法:根据流式细胞仪检测结果将46例未经治疗的初诊急性髓性白血病患者分为四组:仅表达CD40的患者组(①组)、表达CD40+CD40L+OX40-的患者组(②组)、三种抗原全部表达组(③组)和全部不表达组(④组),利用RT-PCR方法检测46例患者骨髓单个核细胞Bcl-2、Bcl-xLmRNA的表达,利用SPSS统计软件分析。结果:①组、④组Bcl-2、Bcl-xL的表达水平低于②、③组的表达水平。结论:在CD40与CD40L同时表达时,可以提高白血病细胞Bcl-2和Bcl-xL的表达。
Objective: To investigate the relationship between the expression of CD40, CD40L and OX40 antigen and the expression of Bcl-2 and Bcl-xL in acute myeloid leukemia (AML). Methods: According to the results of flow cytometry, 46 untreated patients with newly diagnosed acute myeloid leukemia were divided into four groups: (1) patients expressing only CD40 and (2) patients expressing CD40 + CD40L + OX40- The expression of Bcl-2 and Bcl-xL mRNA in bone marrow mononuclear cells of 46 patients was detected by RT-PCR. The expression of Bcl-xL mRNA in bone marrow mononuclear cells was detected by SPSS statistical software analysis. Results: ① The expression levels of Bcl-2 and Bcl-xL in groups 4 and 4 were lower than those in groups ② and ③. Conclusion: The expression of Bcl-2 and Bcl-xL in leukemic cells can be increased when CD40 and CD40L are expressed simultaneously.