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目的：对大鼠离体心脏进行Ｌａｎｇｅｎｄｏｆｆ灌流，观察油茶皂甙（ＳＱＳ）对心肌缺氧／复氧（Ｉ／Ｒ）损伤的保护作用。方法：灌流开始后，将心脏随机分为４ 组。Ｎｏｒｍ ａｌ组：有氧灌流；缺氧／复氧（Ｉ／Ｒ）组：缺氧４０ ｍ ｉｎ 复氧３０ ｍ ｉｎ；ＳＱＳ大剂量组（ＳＱＳＩ）：ＳＱＳ０５ ｍ ｇ／Ｌ预灌１５ ｍ ｉｎ，作Ｉ／Ｒ模型；ＳＱＳ小剂量组（ＳＱＳⅡ）：ＳＱＳ０２５ ｍ ｇ／Ｌ预灌１５ ｍ ｉｎ，作Ｉ／Ｒ模型。记录心功能，测量心肌酶活性。结果：ＳＱＳⅠ、Ⅱ组与Ｉ／Ｒ组相比，ＳＱＳ能增加心肌的收缩功能，提高心室最大收缩压（ＬＶＳＰ），左室内压最大变化速率（ｄｐ／ｄｔｍａｘ），加快心率（ＨＲ）；使冠脉流出液肌酸激酶（ＣＫ）生成减少；降低心肌组织钙含量，增强细胞膜Ｎａ＋ Ｋ＋ ＡＴＰａｓｅ 及线粒体膜Ｃａ２＋ ＡＴＰａｓｅ活性。结论：ＳＱＳ具有抗钙超载及心肌保护作用。
OBJECTIVE: To perform Langendoff perfusion on isolated rat heart and observe the protective effect of Camellia saponin (SQS) on myocardial hypoxia/reoxygenation (I/R) injury. METHODS: After perfusion was started, hearts were randomly divided into 4 groups. Norm al group: aerobic perfusion; hypoxia/reoxygenation (I/R) group: hypoxia 40 m in reoxygenation 30 m in; SQS high-dose group (SQSI): SQS0 5 m g/L pre- irrigation 15 m In, for I/R model; SQS low dose group (SQSII): SQS025 m g/L pre-injection 15 m in for I/R model. Heart function was recorded and myocardial enzyme activity was measured. RESULTS: Compared with the I/R group, SQSI and SQS group increased myocardial systolic function, maximal ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure (dp/dtmax), and accelerated heart rate (HR); Coronary effluent creatine kinase (CK) production decreased; reduced myocardial calcium content, enhanced membrane Na + K + ATPase and mitochondrial membrane Ca 2+ ATPase activity. Conclusion: SQS has anti-calcium overload and myocardial protection.