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采用RT-PCR和RACE技术从奥林达夏橙[Citrus sinensis(L.)cv.Olinda]果萼离层中分离出1个低氧应答基因,命名为CsHRP.CsHRP基因的序列分析推测其编码98个氨基酸残基.CsHRP基因组序列与cDNA比对结果表明其含有2个内含子.Blastp分析发现,CsHRP含有保守的低氧应答保守结构域HIG_1_N,与可可、玉米、橡胶树等植物中的同源蛋白相似度达68%~81%.亚细胞定位显示CsHRP是细胞膜/细胞壁锚定蛋白.qRTPCR试验结果显示CsHRP在幼苗子叶中的表达量明显高于根、茎、叶.在逆境处理条件下,CsHRP表达受低温,高盐,PEG6000,脱落酸,乙烯,甲基茉莉酸和水杨酸诱导,说明该基因响应多种信号转导.
One hypoxia response gene was isolated from the calyx of Citrus sinensis [Citrus sinensis (L.) cv. Olinda] by RT-PCR and RACE, and named as CsHRP. Sequence analysis of CsHRP gene suggested that it encoded 98 amino acid residues.Comparison of the cDNA sequence of CsHRP with cDNA showed that it contains two introns.Blastp analysis found that CsHRP contains conserved hypoxia response conserved domain HIG_1_N, and cocoa, corn, rubber trees and other plants in the same The similarity of the source proteins was 68% -81%. The subcellular localization of CsHRP was the membrane / cell wall anchoring protein.The results of qRTPCR showed that the expression of CsHRP in seedling cotyledons was significantly higher than that in roots, stems and leaves. , CsHRP expression was induced by low temperature, high salt, PEG6000, abscisic acid, ethylene, methyl jasmonic acid and salicylic acid, indicating that the gene responds to various signal transduction.