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组织块法培养兔腹主动脉血管平滑肌细胞,并对其细胞来源进行鉴定。用4.3×106CFU/mL的Pg上清液刺激细胞12、24、48h后,通过ELISA检测IL-1β、IL-6的水平;同时用RT-PCR检测其mRNA表达的情况。结果:Pg上清液刺激24h和48h时能促进血管平滑肌细胞分泌IL-6,分别与同一时间点的对照组相比差异均有统计学意义(P<0.05),而且24h时,IL-6表达最强,而IL-1β的表达最低,明显低于相同时间的对照组和其他时间点的实验组(P<0.05)。RT-
Rabbit abdominal aorta vascular smooth muscle cells were cultured by tissue block method and their cell sources were identified. The cells were stimulated with 4.3 × 106CFU / mL Pg supernatant for 12, 24 and 48 hours. The levels of IL-1β and IL-6 were detected by ELISA. Meanwhile, the mRNA expression was detected by RT-PCR. Results: Pg supernatant stimulated the secretion of IL-6 by vascular smooth muscle cells 24h and 48h, respectively, compared with the control group at the same time point (P <0.05), and at 24h, IL-6 The expression was the strongest, while the expression of IL-1β was the lowest, which was significantly lower than the control group at the same time and the experimental group at other time points (P <0.05). RT-