目的 :研究脑内原发性恶性淋巴瘤临床病理、免疫组化及克隆性基因重排特征。方法 :对 6例脑原发性恶性淋巴瘤作了临床病理形态观察和免疫组化分析 (S P法 ) ,并用PCR技术单轮及半巢式扩增法进行IgH及TCR β基因重排检测。结果 :6例皆为非霍奇金B细胞淋巴瘤。瘤细胞大部分是中心或中心母细胞型 ,常伴浆样分化 ,往往围绕血管排列。PCR克隆性基因重排 ,IgH单轮法 (2 / 6例 )阳性 ,半巢式 (6 / 6例 )阳性 ,TCR β皆为阴性。结论 :脑淋巴瘤有其独特的临床病理特征。免疫组化及基因重排是诊断脑淋巴瘤重要工具。基因重排半巢式比单轮法优越 ,阳性检出率高 Objective: To study the clinicopathological, immunohistochemical and clonal gene rearrangements of primary malignant lymphoma in the brain. Methods: Six cases of primary malignant lymphoma were observed by immunohistochemistry and immunohistochemistry (SP method). IgH and TCR β gene rearrangements were detected by single and half nested PCR with PCR. Results: All 6 cases were non-Hodgkin’s B-cell lymphoma. Most of the tumor cells are centroblasts or centroblasts, often with plasma-like differentiation, often arranged around the blood vessels. PCR clonal gene rearrangement, IgH single wheel method (2/6 cases) positive, semi-nested (6/6 cases) positive, TCR β are negative. Conclusion: Brain lymphoma has its unique clinicopathological features. Immunohistochemistry and gene rearrangement are important tools for diagnosis of brain lymphoma. Gene rearrangement semi-nested superior than the single wheel method, the positive detection rate