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通过试验研究,初步建立起以铁皮石斛种子做外植体的组培快繁体系。铁皮石斛种子萌发适宜的培养基配方为MS+NAA 0.5 mg/L+活性炭0.05%+琼脂0.50%;原球茎增殖分化培养基为MS+NAA 0.5 mg/L+香蕉泥8.00%+琼脂0.50%,生根培养基为1/MS+NAA 0.2 mg/L+香蕉泥8.00%+活性炭0.05%+琼脂0.5%,生根率达100.00%。
Through the experimental study, the rapid tissue culture system was established with explants of Dendrobium candidum as the explant. The optimal medium for the germination of Dendrobium candidum was MS + NAA 0.5 mg / L + activated carbon 0.05% + agar 0.50%; the proliferation and differentiation of protocorms were MS + NAA 0.5 mg / L + banana puree 8.00% + agar 0.50% Base was 1 / MS + NAA 0.2 mg / L + banana puree 8.00% + activated carbon 0.05% + agar 0.5%, rooting rate of 100.00%.