论文部分内容阅读
建立了高效液相色谱法检测人胃黏膜组织中的左氧氟沙星。共收集我院门诊37例幽门螺杆菌(Helicobacter pylori,Hp)阳性的慢性胃炎患者,用含左氧氟沙星三联方案(埃索美拉唑20 mg+阿莫西林1 000 mg,2次/天,左氧氟沙星500 mg,1次/天)根除Hp,疗程7 d。受试者于疗程结束后接受胃镜检查,胃窦部取材3块并制成匀浆,利用HPLC法检测人胃黏膜组织中的左氧氟沙星。采用Hypersil ODS C18色谱柱,流动相为乙腈∶50 mmol/L枸橼酸溶液∶1 mol/L乙酸铵溶液(27∶72∶1),检测波长295 nm。人胃黏膜组织中左氧氟沙星在0.5~10g/g范围内线性关系良好,低、中、高浓度质控样品的方法回收率分别为96.4%、95.2%和102.8%;日内、日间RSD分别为6.70%、2.01%、1.46%和7.40%、4.56%、3.62%。37例患者胃黏膜中左氧氟沙星浓度为(6.05±2.47)μg/g。
A high performance liquid chromatography (HPLC) was established for the determination of levofloxacin in human gastric mucosa. A total of 37 patients with Helicobacter pylori (Hp) -positive chronic gastritis were enrolled in our hospital. The patients were treated with levofloxacin triple therapy (esomeprazole 20 mg + amoxicillin 1000 mg twice daily, levofloxacin 500 mg , 1 times / day) eradicate Hp, course of treatment 7 d. Subjects underwent gastroscopy at the end of the course of treatment. The gastric antrum was sampled and homogenized, and the levofloxacin in human gastric mucosa was detected by HPLC. Using Hypersil ODS C18 column, the mobile phase was acetonitrile: 50 mmol / L citric acid solution: 1 mol / L ammonium acetate solution (27:72:1) and the detection wavelength was 295 nm. The linearity of levofloxacin in human gastric mucosa tissue was good in the range of 0.5-10 g / g, and the recoveries of low, middle and high quality control samples were 96.4%, 95.2% and 102.8%, respectively. The intra-day and interday RSD were 6.70 %, 2.01%, 1.46% and 7.40%, 4.56%, 3.62%. The concentration of levofloxacin in gastric mucosa of 37 patients was (6.05 ± 2.47) μg / g.