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５例健康受试者肌内注射硫酸丁胺卡那霉素（４ｍｇ／Ｋｇ），１０４份血清样品分别经微生物法和荧光偏振免疫法测定丁胺卡那霉素血清药物浓度，结果表明荧光偏振免疫法测定结果显著高于微生物法的测定结果（Ｐ＜０．０１）。两组结果间具有良好的相关性，线性方程为：Ｙ＝１．７７５５＋０．９４９７Ｘ（γ＝０．９９６８），但微生物法在较低浓度时测定误差较大，精密度比荧光偏振免疫测定法低。本实验结果提示采用该两种方法均可对丁胺卡那霉素进行血药浓度监测。
Five healthy subjects were injected intramuscularly with kanamycin sulfate (4mg / Kg) and 104 serum samples respectively. The serum drug concentration of amikacin was determined by microbiological method and fluorescence polarization immunoassay. The results showed that fluorescence polarization Immunoassay results were significantly higher than the results of microbial assay (P <0.01). There was a good correlation between the two sets of results. The linear equation was: Y = 1.7755 + 0.9497X (γ = 0.9968). However, the error of microbiological assay was higher at lower concentration and the precision was higher than that of fluorescence polarization immunoassay low. The results suggest that the use of both methods can be amikacin for plasma concentration monitoring.