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为研究脂多糖(lipopolysaccharide,LPS)对小鼠认知功能的影响,本研究将18只C57BL/6小鼠随机分为两组,即正常对照组和LPS处理组,每组各9只小鼠。在腹腔注射LPS 24 h后行水迷宫实验检测小鼠行为学变化。此外,本研究采用Western blotting和免疫组化检测小鼠脑内小胶质细胞特异性标记物离子通道相关钙衔接蛋白(ionized calcium binding adapter,IBA1)的表达,TUNEL凋亡法检测小鼠脑内神经元凋亡情况。实验结果发现LPS处理后小鼠认知功能下降。Western blotting和免疫组化结果均提示LPS处理后小鼠脑中IBA1表达量增加,小胶质细胞激活;TUNEL提示LPS处理后小鼠脑内出现大量凋亡神经元,由此推断LPS可能通过激活小胶质细胞,扩大神经炎症反应,增加神经元凋亡导致小鼠认知功能下降。
In order to study the effect of lipopolysaccharide (LPS) on cognitive function in mice, 18 C57BL / 6 mice were randomly divided into two groups: normal control group and LPS-treated group, with 9 mice in each group . The rats were subjected to water maze test 24 h after intraperitoneal injection of LPS for behavioral changes. In addition, Western blotting and immunohistochemistry were used to detect the expression of ion channel-dependent calcium binding adapter (IBA1) in mouse brain microglia-specific marker. TUNEL assay was used to detect the intracerebral Neuronal apoptosis. The experimental results showed that cognitive function of mice decreased after LPS treatment. Western blotting and immunohistochemistry showed that the expression of IBA1 in brain increased and microglia activated after LPS treatment. TUNEL suggested that a large number of apoptotic neurons appeared in the brain of LPS-treated mice, Microglia, expansion of neuroinflammation, increased neuronal apoptosis lead to cognitive decline in mice.