最近的动物研究表明,由编码乙型肝炎病毒（HBV）和流感病毒抗原的基因序列制备的“裸”质粒DNA疫苗能激发持久的强免疫应答,从而使研究人员推测,对这种方法进行人体临床试验已为期不远了。 由渥太华和巴黎研究人员组成的一个跨大西洋研究小组构建了携带HBsAg编码基因的质粒,含有S序列（主要蛋白）、前S2和S序列（中蛋白）和前S1、前S2和S序列（大蛋白）。将这些质粒体外转染鼠成肌细胞后,就能合成并分泌这些HBV包膜 Recent animal studies have shown that “naked” plasmid DNA vaccines prepared from gene sequences encoding hepatitis B virus (HBV) and influenza virus antigens can elicit long-lasting strong immune responses, leading researchers to speculate that this approach to human Clinical trials are not far off. A transatlantic team of researchers from Ottawa and Paris constructed a plasmid carrying the HBsAg-encoding gene, containing the S sequence (major protein), the preS2 and S sequences (mesoderm) and the preS1, preS2 and S sequences protein). After transfection of these plasmids into murine myoblasts in vitro, these HBV envelopes can be synthesized and secreted