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应用酶联抗原直接包被法(ELISA—DAC)对4个种传率高低不同的花生品种,苗期感染花生条纹病毒(PStV)的病株在荚果发育不同时期对荚果各部位带毒率进行测定。花生荚壳、胚和子叶都带有PStV病毒,种皮极少带毒。荚壳带毒率变化范围大,规律不明显;种皮带毒偶而能检测到,以上品种间无明显差异。胚和子叶一般同时带毒,带毒率在0—23.8％之间,品种间有明显差异,每个品种带毒率基本不变。荚果各部位带毒率年度间有些变化。
Strains of peanut streak virus (PStV) infected by seedlings of four species with different transmission rates were tested for their virulence in different parts of the pod at different stages of pod development by enzyme-linked antigen direct coating (ELISA-DAC) Determination. Peanut pods, embryos and cotyledons are all with PStV virus, the seed coat with little poison. Pod shell with a wide range of changes in the rate of poisoning, the law is not obvious; kind of belt poison can be detected even more than the above varieties no significant difference. Embryo and cotyledons are generally poisonous at the same time, with a rate of 0-23.8%. There are obvious differences among varieties, and the rate of lethality in each species is basically unchanged. There are some changes in the incidence of pod in various parts of the year.