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本文建立了检测抗PHSA(多聚人血清白蛋白)的醋纤膜快速酶标电泳法,结果较普通对流免疫电泳法(CEP)敏感。 一、测定方法:将醋纤膜切成3×8cm条,在pH8.6、0.05M巴比妥缓冲液中充分浸湿后,吸去多余缓冲液,架于两极用单层棉布搭桥的电泳槽内。用5.5mm 的3号层析滤纸片浸湿待测血清,贴于阳极端(纸片间隔 1.5～2mm)接通电源,开始以110V电压电泳15分钟(夏季100V即可)。再取2.5～3mm宽的普通滤纸条,均匀浸湿辣根过氧化物酶标记抗原(HRP-PHSA,武汉市七医院产品),贴于醋纤膜
In this paper, a rapid enzyme-linked gel electrophoresis method was developed for the detection of anti-PHSA (poly-serum albumin). The results were more sensitive than conventional convective immuno-electrophoresis (CEP). First, the determination of the method: the vinegar membrane cut into 3 × 8cm strips, pH8.6, 0.05M barbiturate buffer fully wet, take away the excess buffer, stand in the poles with a single layer of cotton bypass electrophoresis Inside the tank. With 5.5mm No. 3 chromatography filter paper soaked in serum to be tested, affixed to the anode (paper interval 1.5 ~ 2mm) connected to the power supply, start electrophoresis at 110V for 15 minutes (summer 100V can be). Then take 2.5 ~ 3mm wide ordinary filter paper, evenly soaked horseradish peroxidase labeled antigen (HRP-PHSA, Wuhan City, seven hospital products), affixed to the vinegar membrane