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从短枝停长到花诱导完成期间,分析了金冠苹果幼树短枝顶芽的RNA和DNA含量。结果表明:在花诱导期间,顶芽RNA含量呈双峰曲线,DNA含量呈单峰曲线,且DNA峰在RNA双峰之间;主干环切处理明显地提早和增加成花,使RNA水平在早期有所下降,提早和提高了RNA第二峰,还使DNA水平略有降低,RNA第一峰可能是生长点接受成花信息的最初反映;DNA峰标志着细胞的旺盛分裂和信息转录;RNA第二峰控制着成花的多少和迟早,是花诱导完成的决定因子;这次RNA峰过后一周,花诱导即告完成。
During the period from spur-to-spike induction to flower induction, the contents of RNA and DNA in the shoots of Golden Delicious apple seedlings were analyzed. The results showed that during the induction of flower, the content of top bud RNA showed a bimodal curve, the content of DNA showed a single peak curve, and the peak of DNA was located between two peaks of RNA. The main circumcision significantly increased the flowering and the flowering of RNA, Early decline, early and improve the second peak of RNA, but also to a slight decrease in DNA levels, the first peak of RNA may be the first reflection of the flowering point to accept flowering information; DNA peak marked cell division and information transcription; The second peak of the RNA controls how much and how soon the flower is made and is the deciding factor for the completion of the flower induction; the flower induction is completed one week after this RNA peak.