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目的研究血管紧张素AT1 亚型受体拮抗剂洛沙坦(losartan,LT)对血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞c_fos表达及细胞内游离钙变化的影响。方法斑点杂交及Fura_2技术。结果AngⅡ10nmol/L能诱导培养乳鼠心肌细胞c_fos的一过性高表达及[Ca2 + ]的显著升高。LT20μmol/L能显著抑制Ang Ⅱ诱导的心肌细胞c_fos的高表达 ,而血管紧张素AT2 亚型受体拮抗剂PD123319则无明显作用。LT1～100μmol/L能剂量依赖性地抑制AngⅡ引起的[Ca2 + ]i升高。结论Ang Ⅱ诱导的心肌细胞c_fos高表达及[Ca2 + ]i升高可能是通过AT1 亚型受体介导而实现的 ,LT可能是一种治疗心肌肥厚的有效药物
Objective To investigate the effect of losartan (Angiotensin Ⅱ receptor antagonist angiotensin Ⅱ type 1 receptor antagonist) on c_fos expression and intracellular free calcium in cardiomyocytes induced by angiotensin Ⅱ (AngⅡ). Methods Dot blot hybridization and Fura_2 technology. Results Ang Ⅱ 10nmol / L induced c-fos overexpression and [Ca2 +] in cultured neonatal rat cardiomyocytes. LT20μmol / L could significantly inhibit the high expression of c_fos induced by Ang Ⅱ, while there was no significant effect of angiotensin Ⅱ type 2 receptor antagonist PD123319. LT1 ~ 100μmol / L dose-dependently inhibited Ang Ⅱ-induced [Ca2 +] i increased. Conclusions Ang Ⅱ-induced high c_fos expression and increase of [Ca2 +] i in cardiomyocytes may be mediated through AT1 subtype receptors, and LT may be an effective drug for the treatment of cardiac hypertrophy