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目的探讨幼鼠惊厥持续状态(status convulsion,SC)后癫痫发生过程中胼胝体内有髓神经纤维(myelinated fibers,MF)的变化。方法选用21 d SD幼鼠122只,按随机数字表法分为对照组(n=48)及实验组(n=74)。实验组采用氯化锂匹罗卡品制备SC模型,应用无线遥测脑电图记录SC后癫痫发生不同时期脑电图变化;Gallyas银染观察胼胝体MF形态变化;Western blot检测胼胝体内髓鞘碱性蛋白(myelin basic protein,MBP)含量变化;透射电镜观察MF超微结构的改变。结果 SC后不同时期脑电图的表现符合癫痫发生各阶段的变化;幼鼠SC后癫痫发生的慢性早期及慢性期胼胝体厚度明显变薄;与同龄对照组相比,幼鼠胼胝体内的MBP表达于SC后急性期即开始下降(P<0.05),且潜伏期、慢性早期、慢性期均显著低于正常对照组(P<0.01);透射电镜可见大鼠SC后急性期胼胝体内MF开始出现分层、肿胀,慢性早期及慢性期还可见空泡化。结论幼鼠SC所致胼胝体内MF的损伤贯穿于SC后癫痫发生整个过程。
Objective To investigate the changes of myelinated fibers (MF) in corpus callosum during the onset of epilepsy in juvenile rats after status convulsion (SC). Methods Totally 122 21-day-old SD pups were randomly divided into control group (n = 48) and experimental group (n = 74). In the experimental group, SC model was made by using lithium pilocarpine, electroencephalogram of wireless telemetry was used to record the changes of electroencephalogram (EEG) in different stages of epilepsy. The morphological changes of MF of the corpus callosum were observed by Gallyas silver staining. The myelin basicity (Myelin basic protein, MBP) content changes; transmission electron microscopy MF ultrastructure changes. Results The EEG at different stages after SC was in accordance with the changes of epilepsy at each stage. The thickness of corpus callosum in early and chronic epilepsy was significantly thinner than that of the control group at the same age. The expression of MBP in the corpus callosum (P <0.05), and the latent period, chronic early and chronic period were significantly lower than that of the normal control group (P <0.01). Transmission electron microscopy showed that the MF of the corpus callosum began to appear in acute stage after SC in rats Layer, swelling, chronic early and chronic emptying can be seen. Conclusion The damage of MF in corpus callosum induced by SC in young rats runs through the whole process of epilepsy after SC.