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目的:研究锦灯笼提取物对抗菌药物致小鼠肠道菌群失调的预防作用。方法:48只BALB/c小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、丽珠肠乐(2 mg/kg)组与锦灯笼提取物高、中、低剂量(80、40、20 mg/kg)组,灌胃给药,每天1次,连续7 d。末次给药后第2天灌胃盐酸左氧氟沙星(1.3 mg/kg),每天1次,连续7 d以致小鼠肠道菌群失调。收集小鼠粪便,提取基因组DNA,采用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)法获得肠道菌群指纹图谱,进行相似性及优势条带的序列分析。结果:灌胃抗菌药物前,小鼠肠道优势菌型为内普雷沃氏菌和乳酸杆菌,高、中、低剂量锦灯笼提取物可增加其乳酸杆菌数量;灌胃抗菌药物后,高、中、低剂量锦灯笼提取物可促进模型小鼠肠道内拟杆菌的生长,使其成为优势菌型。结论:锦灯笼提取物能增加肠道乳酸杆菌的数量,对抗菌药物致小鼠肠道菌群失调有预防作用。
Objective: To study the preventive effects of extract of Jin lantern on intestinal microflora in mice induced by antibacterial drugs. Methods: Forty-eight BALB / c mice were randomly divided into normal control (normal saline) group, model (isosteration saline) group, Lizhu Renault (2 mg / kg) Medium and low dose (80,40,20 mg / kg) group, intragastric administration, once a day for 7 days. Levofloxacin hydrochloride (1.3 mg / kg) was given to the mice on the second day after the last administration, once a day for 7 consecutive days to cause imbalance of intestinal microflora in mice. The feces of mice were collected and the genomic DNA was extracted. The gut microarray fingerprint was obtained by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and the similarity and dominant bands were sequenced. Results: Before inoculation of antibacterial drugs, the predominant intestinal flora in mice was Neisseria gonorrhoeae and Lactobacillus. The high, medium and low doses of Jin lantern extract increased the number of lactobacilli. After administration of antimicrobial agents, Medium and low doses of Jin lantern extract can promote the growth of Bacteroidetes in the intestinal tract of model mice, making it a dominant bacterium. Conclusion: Jin lantern extract can increase the number of intestinal Lactobacillus, which can prevent the imbalance of intestinal flora in mice induced by antibacterial drugs.