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目的建立饮用水及水源水中土臭素和2-甲基异莰醇的液液萃取气相色谱-质谱联用测定方法。方法土臭素和2-甲基异莰醇经过正己烷液液萃取、浓缩、进样,经气相色谱-质谱联用法分析测定,选择离子模式定量。结果该方法分离效果好,土臭素和2-甲基异莰醇的浓度为2.5 ng/L~100.0 ng/L时,线性关系良好,线性方程分别为y=1 335.72x+390.6(r=0.999 2)和y=1 130.80x+287.8(r=0.999 3),检出限分别为1.0 ng/L和2.0 ng/L。标准样品添加量为10.0 ng、20.0 ng、50.0 ng时,方法的加标回收率为83%~94%,相对标准偏差(RSD)为3.0%~4.9%。对待测组分在水中的稳定性进行了研究,样品于4℃保存7 d后仍可获得较准确的结果。结论该方法简便,灵敏度高,可用于饮用水及水源水中土臭素和2-甲基异莰醇的测定。
Objective To establish a liquid chromatography-mass spectrometry (LC-MS / MS) method for the determination of geosmin and 2-methylisoxanthene in drinking water and drinking water. Methods Geosmin and 2-methylisoxanthenol were extracted by n-hexane liquid-liquid extraction, concentrated, injected and analyzed by gas chromatography-mass spectrometry. The ion mode was selected for quantification. Results The separation efficiency of this method was good. The linear relationship was obtained when the concentration of geosmin and 2-methylisoxanthene was between 2.5 ng / L and 100.0 ng / L. The linear equations were y = 1 335.72x + 390.6 (r = 0.999 2) and y = 1 130.80x + 287.8 (r = 0.999 3). The limits of detection were 1.0 ng / L and 2.0 ng / L, respectively. The spiked recoveries were 83% ~ 94% and the relative standard deviations (RSDs) were 3.0% ~ 4.9% for standard samples of 10.0 ng, 20.0 ng and 50.0 ng respectively. The stability of the tested components in water was studied. After the samples were stored at 4 ℃ for 7 days, more accurate results were obtained. Conclusion The method is simple and sensitive, and can be used for the determination of geosmin and 2-methylisoxrenol in drinking water and drinking water.