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为了有助于阐明人生长素在正常乳腺机能或人类乳腺疾病中是否具有生理意义,作者用灵长类动物检查了人生长素的促乳活性。从7只雌恒河猴,6只雌短尾猴及1只雄性狒狒得到乳腺组织用于器官培养。连续培养9天,每3天换1次培养液。在199培养液中加入妇女血清,胰岛素(10μg/ml)及氢化考的松(20μg/ml)。以培养条件下乳腺组织的α-乳清蛋白产生量为指标,观察并比较了人生长素和羊催乳素的促乳活性。使加入培养环境中的人生长素和羊催乳素保持等浓度,在恒河猴及狒狒约为30～1,000 ng/ml,而用于短尾猴乳腺培养为100～5,000ng/ml。每份
To help elucidate the physiological significance of human growth hormone in normal breast function or in human breast disease, the authors examined the stimulant activity of human growth hormone using primates. Breast tissues were obtained from seven female rhesus monkeys, six cynomolgus monkeys and one male baboon for organ culture. Continuous culture for 9 days, every 3 days for 1 medium. Women’s serum, insulin (10 μg / ml) and hydrocortisone (20 μg / ml) were added to the culture broth. The production of α-lactalbumin in breast tissue was used as an indicator to observe and compare the promoting activities of human growth hormone and sheep prolactin. Equal concentrations of human growth hormone and ovine prolactin were added to the culture environment at about 30 to 1,000 ng / ml in rhesus macaques and baboons, and 100 to 5,000 ng / ml in mammary glands for cynomolgus monkeys. Each