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为研究宿主细胞和寄生菌的相互关系,作者研究了几种细菌对鼻粘膜细胞的粘附能力及细菌在鼻粘膜上的分布。以压舌板刮取法,收集2～5个正常人前鼻腔鼻粘膜细胞,放在薄膜上(孔径14毫微米),用磷酸缓冲液洗,以去除分泌物与未粘附细菌,作细胞计数,调整细胞浓度至10~4～10~5/毫升,分别与浓度为10~8/毫升的几种细菌悬液等量混合,35℃孵育90分钟后,用磷酸缓冲液洗去未粘附的细菌,做涂片染色,镜检计数附在细胞上细菌数目,结果见表。作者还试验了
To study the relationship between host cells and parasites, the authors studied the adhesion of several bacteria to nasal mucosal cells and the distribution of bacteria on the nasal mucosa. With pressure Tongue scraping method to collect 2 to 5 normal nasal nasal mucosa cells, on the membrane (14 nm pore size), washed with phosphate buffer to remove secretions and non-adhesion bacteria for cell counting, Adjust the cell concentration to 10 ~ 4 ~ 10 ~ 5 / ml, respectively, with the concentration of 10 ~ 8 / ml several bacterial suspensions mixed equal 35 minutes after incubation for 90 minutes, washed with phosphate buffer unadhered Bacteria, smear staining, microscopic count attached to the number of bacteria on the cells, the results shown in the table. The author also tested