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目的探索黄芩扦插生根技术。方法配制不同浓度的IAA和NAA,浸泡取自不同位置的黄芩插穗3 h,再扦插于蛭石与砂的混合基质中,室温下培育使其生根,定期记录并统计插穗生根状况,最后分析实验数据。结果黄芩插穗切口处在激素诱导下膨大并生出根;不同浓度的IAA(吲哚乙酸)和NAA(萘乙酸)处理插穗,IAA可促进诱导插穗生根,它对黄芩茎梢生根的促进作用优于NAA;IAA50 mg/L时生根率较高,插穗平均生根率达65.0%,比对照高42.5%;就不同切取位置:IAA100 mg/L时30.0%(节上)>0(节间),IAA50 mg/L时75.0%(节上)>55.0%(节间),IAA25 mg/L时50.0%(节上)>40.0%(节间),即从节上剪切的插穗的生根能力强于从节间切取的。结论通过一系列的幼苗扦插探究实验,初步建立起黄芩幼苗扦插培育体系,指导黄芩扦插技术的实施。
Objective To explore the rooting technique of Scutellaria baicalensis. Methods Different concentrations of IAA and NAA were prepared and soaked in Scutellaria baicalensis Georgi at different positions for 3 h. Then they were cut into mixed medium of vermiculite and sand, and were incubated at room temperature for rooting. The rooting status of cuttings was recorded and counted regularly. Finally, data. Results Scutellariae cuttings were inflated under hormones and rooted. Different concentrations of IAA (IAA) and NAA (naphthaleneacetic acid) cuttings, IAA could promote the induction of rooting, and it had a better effect on rooting of stems of Scutellaria baicalensis NAA; the rooting rate of IAA was higher at 50 mg / L IAA, the average rooting rate of cuttings was 65.0%, which was 42.5% higher than that of the control. For different cut locations: 30.0% 75.0% (internodes) and 55.0% (internodes) at mg / L and 50.0% (on nodes)> 40.0% (internodes) at 25 mg / L of IAA, ie, the cuttings cut from nodes had stronger rooting ability Cut from internode Conclusion Through a series of seedling cutting experiments, the initial cultivation of Scutellaria baicalensis seedlings was established to guide the implementation of Scutellaria baicalensis cuttings.