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目的：对人抗甲肝病毒噬菌体抗体阳性克隆５９７和６４６，进行重链ＤＮＡ序列分析。方法：用Ｓａｎｇｅｒ末端终止法测定序列，计算机软件进行分析。结果：经同源性分析，这两个克隆的同源基因均来源于人免疫球蛋白重链基因序列。氨基酸序列推导可知，克隆６４６重链Ｆｄ基因和克隆５９７重链可变区基因均为单一开放读框，各编码２２５个氨基酸和１１９个氨基酸。２株克隆重链可变区含有明确的四个框架区和３个抗原决定互补区。结论：克隆６４６和克隆５９７的重链ＤＮＡ序列具有典型的人免疫球蛋白基因序列特征
Objective: To analyze the heavy chain DNA sequence of human anti-Hepatitis A virus phage antibody positive clones 597 and 646. Methods: The sequences were determined by Sanger terminal method and analyzed by computer software. Results: Homology analysis showed that the homologous genes of these two clones originated from human immunoglobulin heavy chain gene sequence. Amino acid sequence deduced, the cloned 646 heavy chain Fd gene and clone 597 heavy chain variable region genes are a single open reading frame, each encoding 225 amino acids and 119 amino acids. The two cloned heavy chain variable regions contain four distinct framework regions and three epitopes complementary to each other. Conclusion: The heavy chain DNA sequences of clone 646 and clone 597 have typical human immunoglobulin gene sequence characteristics