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采用SDS-PAGE技术对70株不同来源及致病力青枯雷尔氏菌(Ralstonia solanacearum)进行胞外蛋白指纹多态性分析,研究结果表明,供试青枯雷尔氏菌菌株呈现丰富的胞外蛋白指纹多态性,多态性比率为100%。不同来源青枯雷尔氏菌分泌的胞外蛋白不同,EZ-Tn5~(TM)插入诱变菌株电泳出20条不同大小蛋白条带,分子量集中在20~97 kD,且菌株间蛋白条带相似或完全相同;~(60)Co辐射诱变菌株共电泳出16条不同大小的蛋白条带,多数蛋白分子量44.3 kD;野生型菌株电泳的蛋白条带最多,共获得26条不同大小的蛋白条带。进一步对37株不同致病力的野生型菌株进行胞外蛋白含量测定,结果表明,不同致病力菌株胞外蛋白含量差异大,强致病力菌株分泌的胞外蛋白含量较高,为1.026~5.963μg/mL,无致病力菌株胞外蛋白含量较低,为0.083~0.761μg/mL。
SDS-PAGE was used to analyze the polymorphism of extracellular protein fingerprints of 70 different sources and virulence factors of Ralstonia solanacearum. The results showed that the Ralstonia solanacearum strain was rich in Extracellular protein fingerprinting polymorphism, the rate of polymorphism was 100%. Different sources of Ralstonia solanacearum secretion of different extracellular proteins, EZ-Tn5 ~ (TM) insertion mutagenesis electrophoresis of 20 different size protein bands, the molecular weight concentrated in 20 ~ 97 kD, and strains of protein bands Similar or exactly the same; 60 (60 Co) radiation mutagenesis strains co-electrophoresis 16 different size protein bands, the majority of protein molecular weight 44.3 kD; wild-type strains of electrophoresis protein bands up to a total of 26 different sizes of proteins Bands. Further analysis of 37 extracellular wild-type strains of extracellular protein content, the results showed that different virulence strains of extracellular protein content difference, strong virulence strains secreted extracellular protein content was 1.026 ~ 5.963μg / mL, non-pathogenic strains of extracellular protein content is low, 0.083 ~ 0.761μg / mL.