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目的 观察多肽生长因子中的转化生长因子 β1(TGF β1)、表皮生长因子 (EGF)、碱性成纤维生长因子 (bFGF)、角化生长因子 (KGF)对雄激素非依赖性前列腺癌细胞系PC 3M生长的影响。 方法 体外培养前列腺癌细胞系PC 3M ,分别加入TGF β1、EGF、bFGF、KGF ,每种生长因子采用 1、5、10、5 0ng/ml 4种浓度 ,采用Brdu掺入法测定细胞生长水平。 结果 培养 72h后 ,对照组PC 3M细胞的吸光度〔A ,旧称光密度 (OD)〕值为 0 75 9,4种生长因子在不同浓度时对PC 3M细胞的生长皆有抑制作用 ,而且随浓度的增加抑制作用逐渐增强。 4种因子在 5 0ng/ml时 ,A值分别为 0 40 0 (TGF β1)、0 45 5 (EGF)、0 5 32 (bFGF)、0 491(KGF) ,与对照组比较 ,差异均具有显著性。另外 ,当 1ng/mlEGF分别与 0 5ng/ml或 5ng/mlTGF β1同时加入培养基时 ,A值分别为 0 5 2 2和 0 2 99。 结论 在体外培养条件下 ,TGF β1、EGF、bFGF及KGF均依浓度效应关系抑制PC 3M细胞的生长 ,TGF β1与EGF对PC 3M细胞生长的抑制作用不存在交互作用。
Objective To observe the effects of TGFβ1, EGF, bFGF and KGF on the proliferation of androgen-independent prostate cancer cell lines Impact of PC 3M growth. Methods Prostate cancer cell line PC 3M was cultured in vitro. TGF β 1, EGF, bFGF and KGF were respectively added into the culture. The growth of the cells was determined by Brdu incorporation assay. Results The absorbance (A, OD) of PC 3M cells in control group was 0 75 9 after culture for 72 hours. The growth of PC 3M cells was inhibited by 4 kinds of growth factors at different concentrations, Increasing inhibitory effect gradually increased. When the four factors were at 50ng / ml, the values of A were 0 40 0 (TGFβ1), 0 455 (EGF), 0 5 32 (bFGF) and 0 491 (KGF) respectively. Significance. In addition, when 1 ng / ml EGF was added to the medium simultaneously with 0 5 ng / ml or 5 ng / ml TGFβ1, the A values were 0 5 2 2 and 0 2 99, respectively. Conclusion Under the culture conditions, TGFβ1, EGF, bFGF and KGF all inhibit the growth of PC-3M cells in a concentration-dependent manner. There was no interaction between TGF-β1 and EGF on the growth inhibition of PC-3M cells.