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目的观察p53腺病毒重组体(AdCMV-p53)转染对p53缺失肿瘤细胞辐射敏感性的影响。方法用腺病毒重组体(AdCMV-p53/GFP)转染经0、0.25、0.5、1.0、1.5、2.0Gyγ射线辐射的H1299(nullp53)和PC-3(nullp53)细胞,用流式细胞分析法检测外源性p53表达,用克隆形成法检测肿瘤细胞增殖能力。结果辐射联合AdCMV-p53转染组p53阳性细胞所占比例均明显高于单纯辐射组、单纯AdCMV-p53转染组和辐射联合AdCMV-GFP转染组同种细胞p53阳性率(P<0.05);AdCMV-p53转染不仅明显提高肿瘤细胞辐射敏感性,而且与肿瘤细胞组织来源有关。结论p53腺病毒重组体转染对p53基因缺失肿瘤细胞低剂量辐射敏感性的增强作用与肿瘤细胞来源的组织器官和细胞类型有关。
Objective To investigate the effect of p53 adenovirus vector (AdCMV-p53) transfection on the radiosensitivity of p53-deficient tumor cells. Methods The H1299 (nullp53) and PC-3 (nullp53) cells irradiated with 0,0.25,0.5,1.0,1.5 and 2.0 Gy γ-rays were transfected with AdCMV-p53 / GFP by flow cytometry The expression of exogenous p53 was detected, and the proliferation of tumor cells was detected by clone formation assay. Results The proportion of p53 positive cells in radiation combined with AdCMV-p53 transfection group was significantly higher than that in pure radiotherapy group. The positive rate of p53 in allogeneic cells of AdCMV-p53 transfection group and AdCMV-GFP transfection group (P <0.05) AdCMV-p53 transfection not only significantly increased the radiosensitivity of tumor cells, but also related to the source of tumor cells. CONCLUSION: The enhanced effect of p53 adenovirus transfection on the sensitivity of low-dose radiation to p53-deficient tumor cells is related to the tissues, organs and cell types of tumor cells.