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目的:探讨长链非编码RNA (lncRNA)LOC730101对骨肉瘤U2OS细胞增殖、侵袭和迁移的影响及其机制。方法:2019年2月至2019年10月,将体外培养的U2OS细胞分为对照组(正常培养)、阴性组(转染阴性对照)和干扰组(转染靶向LOC730101的干扰序列),采用RT-PCR检测细胞中LOC730101表达,噻唑蓝(MTT)法检测细胞增殖活力,平板克隆形成实验检测细胞克隆形成率,流式细胞仪检测细胞周期分布情况, Transwell小室检测细胞侵袭与迁移,Western blotting法检测细胞中上皮间质转化相关蛋白波形蛋白(Vi- mentin)、N-钙黏附素(N-cadherin)、E-钙黏附蛋白(E-cadherin)和Wnt/β-连环蛋白(β-catenin)信号通路相关蛋白β-catenin、c-Myc、细胞周期蛋白D1 (Cyclin D1)和基质金属蛋白酶-7 (MMP-7)蛋白的表达水平。结果进行统计学分析,n P<0.05为差异有统计学意义。n 结果:与对照组比较,干扰组细胞中LOC730101表达水平(干扰组、对照组分别为0.25±0.03、1.00±0.06,下同)、细胞成活率[(57.65±3.26)%、(100.00±7.39)%]、克隆形成率[(13.03 ± 2.12)%、(25.35±3.58)%]、侵袭细胞数(51.36±3.48、92.85±6.62)、迁移细胞数(77.15 ± 5.05、136.92 ± 15.35)和细胞在S期[(20.54±2.15)%、(28.15±2.38)%]、Gn 2/M期所占百分比[(16.87±2.12)%、(23.36±3.12)%]以及细胞中Vimentin (0.52 ± 0.04、1.17 ± 0.13)、N-cadherin (0.31 ± 0.03、0.65 ± 0.04)、β-catenin (0.42 ± 0.03、0.73 ± 0.04)、c-Myc (0.29±0.03、0.65±0.03)、Cyclin D1 (0.26±0.02、0.58±0.04)、MMP-7蛋白表达水平(分别为0.55± 0.03、0.86±0.06)均明显降低,而细胞在Gn 0/Gn 1期所占百分比[分别为(62.62±5.15)%、(48.46±3.65)%]以及细胞中E-cadherin蛋白的表达水平(分别为0.82±0.06、0.38±0.03)均明显升高,差异均有统计学意义(n P0.05)。n 结论:下调LOC730101可抑制骨肉瘤U2OS细胞增殖、侵袭和迁移,其作用机制可能与抑制Wnt/β-catenin信号通路有关。“,”Objective:To investigate the effect of long-chain non coding RNA (lncrna) loc730101 in the proliferation, invasion and migration of U2OS cells, and its mechanism.Methods:From February, 2019 to October, 2019, U2OS cells cultured n in vitro were divided into control group (normal culture), negative group (transfection nega- tive control), and interference group (transfection of interference sequences targeting LOC730101). The expression of LOC730101 in cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). Cell proliferation ac tivity was tested by methylthiazolyldiphenyl-tetrazolium bromide (MTT) method. Cell clone formation rate was mearused by plate clone formation test. Cell cycle distribution was tested by flow cytometry. Cell invasion and migra- tion were examed by Transwell chamber. The expression levels of epithelial-mesenchymal transition-related proteins Vimentin, N-cadherin, E-cadherin, and Wnt/β-catenin signaling pathway related proteins in cells β-catenin, c-Myc, cyclin D1(CyclinD1) and matrix metalloproteinase-7(MMP-7) proteins were detected by Western blotting method. The date was statistical analysed and considered as statistically significant whenn P<0.05.n Results:Compared with the control group, the expression level of LOC730101 (0.25±0.03 and 1.00±0.06) in interference group and control group, respectively. The same below), cell survival rate [(57.65±3.26)% and (100.00±7.39)%], clone formation rate [(13.03± 2.12)% and (25.35±3.58)%], number of invasive cells(51.36±3.48 and 92.85±6.62), number of migrating cells (77.15± 5.05 and 136.92±15.35), the percentage of cells in S phase [(20.54±2.15)% and (28.15±2.38)%] and Gn 2/M phase [(16.87±2.12)% and (23.36±3.12)%], as well as the expression level of Vimentin (0.52±0.04 and 1.17±0.13), N-cadherin (0.31±0.03 and 0.65±0.04), β-catenin (0.42±0.03 and 0.73±0.04), c-Myc (0.29±0.03 and 0.65±0.03), CyclinD1 (0.26± 0.02 and 0.58±0.04), MMP-7 protein (0.55±0.03 and 0.86±0.06) was decreased significantly ( n P<0.05), while the per- centage of cells in Gn 0/Gn 1 phase [(62.62±5.15)% and (48.46±3.65)%] and the expression level of E-cadherin protein(0.82± 0.06 and 0.38±0.03) were increased significantly in the interference group (n P0.05).n Conclusion:Reduce the regulation of LOC730101 can inhibit the proliferation, invasion and migration of U2OS cells, and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.