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目的:观察骨形成蛋白(bone morphogenetic protein)特异的细胞内信号转导分子Smadl在人牙乳头细胞内的表达及信号转位过程,从细胞内信号转导水平探讨牙齿发育过程中成牙本质细胞的分化机制。方法:原代培养人牙乳头细胞,用转化生长因子β1(transforming growth factor β1,TGF-β1)和重组人骨形成蛋白骨形成蛋白2(recombinant hu-man bone morphogenetic protein,rhBMP2)刺激培养的细胞,对照组用0.2％FCS的DMEM的培养液培养,不加任何刺激。免疫组化观察。结果:TGF-β1和rhBMP2刺激组均可见Smadl蛋白表达,但与对照组相比无明显差异;rhBMP2组可见Smadl从胞浆转位至核内聚集,TGF-β1组未见此作用。结论:首次观察到Smadl基因在人牙乳头细胞内的表达并参与信号转位过程,提示BMP2在牙乳头细胞内信号传递可能是通过Smadl转位至核内引起基因表达实现的。
OBJECTIVE: To observe the expression and signal transduction of Smadl, a specific intracellular signal transduction molecule of bone morphogenetic protein, in human papillary cells. To explore the role of Smadl in odontoblasts Differentiation mechanism. Methods: Human dental papilla cells were cultured in primary culture, and the cultured cells were stimulated with transforming growth factor β1 (TGF-β1) and recombinant hu-man bone morphogenetic protein (rhBMP2) The control group was cultured with 0.2% FCS in DMEM without any stimulation. Immunohistochemical observation. Results: Smadl protein expression was observed in TGF-β1 and rhBMP2-stimulated groups, but there was no significant difference compared with control group. Smad1 translocated from cytoplasm to nucleus in rhBMP2 group but not in TGF-β1 group. Conclusion: The expression of Smadl gene in human dental papilla cells was observed for the first time and involved in signal translocation process, suggesting that BMP2 signaling in dental papilla cells may be caused by Smadl translocation into the nucleus to cause gene expression.