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近年发现肾间质细胞能分泌一种具有明显降压作用的抗高血压活性物质,称为抗高血压脂,它分为抗高血压极性和中性脂两类。本文介绍显示肾间质细胞(renomedullary interstitial cells,RLCs)及其胞浆中分泌颗粒的固定和染色方法。 1 材料与方法固定液的配制 Levi固定原液:含重铬酸钾2.5 g、升汞5.0 g、蒸馏水100 ml;Levi固定液:将Levi原液、2％锇酸和10％中性福尔马林按5:1:1的比例混合,用前配制。实验动物取10周龄、体重200g左右Wistar大鼠,乙醚麻醉,用02 MPBS配制的2.5％戊二醛,经心脏、主动脉进行灌流固定,取大鼠双侧肾脏,在肾髓质乳头部取材。
In recent years, it has been found that renal interstitial cells can secrete an antihypertensive active substance with a significant antihypertensive effect, called antihypertensive lipids, which are classified into antihypertensive and neutral lipid types. This article presents the methods for the fixation and staining of secretory granules in renal mesenchymal cells (RLCs) and their cytoplasm. 1 Materials and Methods Fixative Solution Preparation of Levi Fix: 2.5 g of potassium dichromate, 5.0 g of mercury, 100 ml of distilled water; Levi’s fixative: Levi’s stock solution, 2% citric acid, and 10% neutral formalin Mix in a ratio of 5:1:1 and prepare before use. Experimental animals Wistar rats of 10 weeks of age and body weight of about 200 g were anesthetized with ether, 2.5% glutaraldehyde in 02 MPBS was used, perfusion fixed with the heart and aorta, and bilateral rat kidneys were taken at the medullary nipple. Draw.