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目的分析江西省2009-2011年甲型H1N1流感病毒M2以及NA基因的特点,掌握其耐药情况,为临床治疗和疾病控制提供参考。方法从江西省流感监测网中随机选择19株甲型H1N1流感病毒,经核酸提取和one-step RT-PCR扩增M以及NA基因片段,双向序列测定,采用DNAStar5.0和Mage4.0序列分析软件分析M2以及NA基因特征以及耐药性位点。结果 19株毒株M2基因发生了S31N的突变;仅A/JiangxiDonghu/SWL11171/2009(H1)株的NA蛋白发生了E119Q替换,其他毒株NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论 19株毒株均对金刚烷胺类药物耐药,几乎都对流感病毒神经氨酸酶抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。
Objective To analyze the characteristics of M2 and NA genes of influenza A (H1N1) virus in Jiangxi province from 2009 to 2011 and to master their resistance to provide a reference for clinical treatment and disease control. Methods 19 influenza A (H1N1) viruses were randomly selected from the influenza surveillance network of Jiangxi Province. The nucleic acids were extracted and one-step RT-PCR was used to amplify the M and NA gene fragments. The DNA sequence was analyzed by DNAStar5.0 and Mage4.0 Software analyzes M2 as well as NA gene signature and drug resistance loci. Results The mutation of S31N occurred in the M2 gene of 19 strains. Only E119Q substitution occurred in the NA protein of A / JiangxiDonghu / SWL11171 / 2009 (H1) strain. No amino acid was found in the NA protein of the other strains replace. Conclusion All the 19 strains were resistant to amantadine drugs and almost all were sensitive to influenza virus neuraminidase inhibitors. However, the surveillance of influenza virus resistance should be strengthened.