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目的探讨射线诱发肾癌细胞凋亡的作用和射线对肾癌细胞Ｐ５３，Ｂｃｌ－２基因表达的影响。方法利用末端脱氧核苷酰转移酶标记的流式细胞技术定性和定量分析射线诱导肾癌细胞凋亡。利用Ｐ５３和Ｂｃｌ－２单抗进行ＳＰＴＭ法免疫组织化学染色。结果０Ｇｙ、５Ｇｙ、１０Ｇｙ、１５Ｇｙ和２０Ｇｙ照射后细胞凋亡量分别为１．０２％、０．０３％、７．０３％、４．２２％和３６．０９％。０Ｇｙ照射组Ｐ５３表达标记指数为０．８０％，２０Ｇｙ照射后Ｐ５３表达明显增加达２３．８０％。０Ｇｙ照射组Ｂｃｌ－２基因表达为２４．４０％，２０Ｇｙ照射后Ｂｃｌ－２基因表达明显减少为１．８０％。结论低剂量Ｘ射线照射诱发肾癌细胞凋亡量很低，２０Ｇｙ时明显增加。射线诱发肾癌细胞凋亡伴随Ｐ５３基因表达增加和Ｂｃｌ－２基因表达减少，提示射线诱发细胞凋亡是多基因相互影响，相互作用的结果。
Objective To investigate the effect of radiation on the apoptosis of renal cell carcinoma and the effect of radiation on the expression of P53 and Bcl-2 in renal cell carcinoma. Methods Flow cytometry using terminal deoxynucleotidyl transferase was used to characterize and quantify the radiation-induced apoptosis of renal cell carcinoma. SPTM immunohistochemical staining using P53 and Bcl-2 mAb. Results The apoptotic rates were 1.02%, 0.03%, 7.03%, 4.22% and 36.09% after 0Gy, 5Gy, 10Gy, 15Gy and 20Gy irradiation, respectively. The expression index of P53 in 0Gy irradiation group was 0.80%, and the expression of P53 increased significantly by 23.80% after 20Gy irradiation. The expression of Bcl-2 gene in the group of 0Gy irradiation was 24.40%, and the expression of Bcl-2 gene in the group of 20Gy irradiation was significantly decreased to 1.80%. Conclusion The apoptosis of renal cancer cells induced by low-dose X-ray irradiation is very low and significantly increased at 20 Gy. RAY-INDUCED APOPTOSIS OF RENAL CARCINOMA CELLS WITH INCREASE OF P53 GENE AND Bcl-2 GENE EXPRESSION INDICATES RAY-INDUCED APOPTOSIS IS A MULTI-GENE INTERACTION AND INTERACTION.