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目的 克隆并分析血链球菌 ATCC10 5 5 6菌株中一种保守蛋白完整基因。方法 利用Southern印迹杂交分析法、分子克隆及斑点杂交技术获得含有特异 DNA片段的目的克隆 ,对该目的克隆测序 ,并利用计算机软件和生物信息学技术对克隆的基因及编码蛋白进行分析。结果 首次发现并克隆了血链球菌 ATCC 10 5 5 6菌株中完整的保守蛋白基因。将其命名为 Sscp基因 ,该基因在 Genbank的登录号 :AY0 32 739。该基因编码一种跨膜蛋白 ,含有两个 AAA结构域。结论 Sscp基因编码蛋白的功能与大肠杆菌 Fts K相似 ,参与血链球菌细胞分裂活动。
Objective To clone and analyze a complete gene of conserved protein in Streptococcus sanguis ATCC 10565. Methods The target clones containing specific DNA fragments were obtained by Southern blotting, molecular cloning and dot blot hybridization. The clones were sequenced and the cloned genes and encoded proteins were analyzed by computer software and bioinformatics techniques. Results The complete conserved protein gene in Streptococcus sanguis ATCC 10565 was first found and cloned. Name it Sscp gene, the gene in Genbank accession number: AY032739. This gene encodes a transmembrane protein containing two AAA domains. Conclusion The function of Sscp gene is similar to that of Fts K in Escherichia coli and is involved in the cell division of Streptococcus sanguis.