作者制备了抗人IgE、IgG_1、IgG_2,IgG_3、IgG_4、IgM和IgA_1的小鼠单克隆抗体并对它们刺激人外周血白细胞释放组织胺的能力进行了研究。杂交瘤抗体是用纯化的不同类型的免疫球蛋白免疫BALB/C小鼠而获得。作者采用非过敏的正常人静脉血加入0.1M EDTA和6%DextranT 500,在室温静置1小时后分离出白细胞,将洗过的细胞 The authors prepared mouse monoclonal antibodies against human IgE, IgG_1, IgG_2, IgG_3, IgG_4, IgM and IgA_1 and studied their ability to stimulate the release of histamine from human peripheral white blood cells. Hybridoma antibodies are obtained by immunizing BALB / C mice with purified different types of immunoglobulins. The authors used non-allergic normal human venous blood was added 0.1M EDTA and 6% DextranT 500, left at room temperature for 1 hour after the separation of leukocytes, the washed cells