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目的 :评价和探讨Fas/FasL抗原在葡萄膜炎患者外周血T淋巴细胞上的表达及其可能存在的意义。方法 :采用流式细胞术 (flowcytometry,FCM)及三色荧光双标记细胞免疫荧光染色技术对 2 6例Behc,et病、17例Vogt 小柳原田综合征患者、2 5例特发性前葡萄膜炎患者及 43例正常人外周血CD4 + T细胞和CD8+ T细胞表面Fas和FasL的表达进行检测。结果 :Behc,et病、Vogt 小柳原田综合征和特发性前葡萄膜炎患者CD4 + T细胞Fas的表达阳性率分别为 (2 5 .70± 7.32 ) %、(19.6 0± 11.0 2 ) %、(2 0 .81± 7.40 ) % ,均显著高于正常对照组[(14.0 2± 6 .30 ) % ],而FasL的表达阳性率与正常对照组比较 ,差异无显著性意义。Behc,et病和Vogt 小柳原田综合征患者CD8+ T细胞Fas的表达阳性率分别为 (9.47± 6 .97) % ,(6 .84± 5 .5 3) % ,均显著高于正常对照组[(3 .47± 2 .75 ) % ];特发性前葡萄膜炎患者CD8+ T细胞Fas的表达阳性率 [(5 .34± 3.0 8) % ]与正常对照组[(3.47± 2 .75 ) % ]比较 ,差异无显著性意义。Behc,et病和Vogt 小柳原田综合征患者CD8+ T细胞FasL的表达阳性率分别为 (3 .6 6± 2 .16 ) %、(4.0 5± 1.38) % ,与正常对照组 [(3.0 9± 2 .0 4) % ]比较 ,差异无显著性意义 ;特发性前葡萄膜炎患者CD8+ T细胞FasL
Objective: To evaluate and explore the expression of Fas / FasL antigen on peripheral blood T lymphocytes in patients with uveitis and its possible significance. Methods: Twenty-six cases of Behc et al disease, 17 cases of Vogt Harassment Harada syndrome and 25 cases of idiopathic uveal uveitis were studied by flowcytometry (FCM) and three-color fluorescence double-labeled cell immunofluorescence staining Inflammatory patients and 43 normal controls. The expressions of Fas and FasL on the surface of CD4 + T cells and CD8 + T cells were detected. Results: The positive rates of Fas in CD4 + T cells in Behc et al disease, Vogt Harassment and idiopathic uveitis were (25.7 ± 7.32)% and (19.6 ± 11.0 2)%, respectively , (20.81 ± 7.40)%, respectively, which were significantly higher than those in the control group [(14.0 ± 2.30)%], while the positive rate of FasL expression was not significantly different from that of the normal control group. The positive rates of Fas expression in CD8 + T cells of patients with Behc et al disease and Vogt Harassment Harassment were (9.47 ± 6.97)% and (6.84 ± 5.53)%, respectively, which were significantly higher than those in the normal control group [ (3.47 ± 2.75)%]. The positive expression rate of Fas in CD8 + T cells in idiopathic uveitis [(5.34 ± 3.08)%] was significantly higher than that in normal control [(3.47 ± 2.75 )%] Comparison, the difference was not significant. The positive rates of FasL expression in CD8 + T cells in Behc et al disease and Vogt Harassment patients were (3.66 ± 2.16)% and (4.05 ± 1.38)%, respectively, which were significantly higher than those in normal control group [(3.09 ± 2 .0 4)%], the difference was not significant; idiopathic uveitis in patients with CD8 T cells FasL