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目的研究表没食子儿茶素没食子酸酯(EGCG)对体外培养食管癌细胞增殖、凋亡和转移的影响及其分子机制。方法采用CCK-8法和细胞克隆形成法检测EGCG对食管癌细胞增殖的影响;流式细胞法检测细胞凋亡及周期分布;Transwell法检测细胞体外侵袭力的改变;免疫印迹法检测葡萄糖调节蛋白78(GRP78)表达水平。结果 EGCG处理后,食管癌细胞的增殖和侵袭能力明显受到抑制,凋亡率也显著增高,呈量效关系;随着EGCG浓度升高,GRP78蛋白表达水平明显下降。结论 EGCG可对食管癌细胞产生抑制增殖、诱导凋亡、降低侵袭和转移等作用,可能与其下调细胞内GRP78蛋白有关。
Objective To investigate the effects of epigallocatechin gallate (EGCG) on the proliferation, apoptosis and metastasis of esophageal carcinoma cells in vitro and its molecular mechanism. Methods The effects of EGCG on the proliferation of esophageal cancer cells were detected by CCK-8 and cell clone formation assay. The apoptosis and cell cycle distribution were detected by flow cytometry. The invasiveness of cells was detected by Transwell assay. The expressions of glucose regulatory protein 78 (GRP78) expression level. Results After EGCG treatment, the proliferation and invasion ability of esophageal cancer cells were significantly inhibited and the apoptosis rate was significantly increased with a dose-response relationship. With the increase of EGCG concentration, the GRP78 protein expression was significantly decreased. Conclusion EGCG can inhibit esophageal cancer cells proliferation, induce apoptosis, reduce the invasion and metastasis, which may be related to its down-regulation of GRP78 protein.