作者1989和1990年用自己新配制的K_3培养基培养春小麦花药,结果其出愈率较用C_(17)培养基分别提高0.3％和4.1％,绿苗分化率提高6.9％和9.7％。K_3培养基还可使春小麦花粉愈伤组织一步成苗,成苗率为22.3％。 In 1989 and 1990, the anther culture of spring wheat with K_3 medium was used. The results showed that the emergence rate of anthers increased by 0.3% and 4.1% respectively compared with that of C_ (17) medium, and the differentiation rate of green plantlets increased by 6.9% and 9.7% respectively. K_3 medium can also make spring wheat pollen callus in one step into seedlings, seedling rate was 22.3%.