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目的：研究我国宫颈癌高发区新疆维吾尔族妇女宫颈癌与人类乳头状瘤病毒（ＨＰＶ）感染的关系。方法：对６５例新疆维吾尔族妇女的宫颈癌活检组织标本，应用原位杂交法检测ＨＰＶ６／１１、１６／１８和３１／３３／３５ＤＮＡ；Ｌ１共有序列引物聚合酶链反应（ＰＣＲ）及Ｅ６特异型引物ＰＣＲ检测其中的５８例ＨＰＶ６、１６和１８ＤＮＡ。结果：６５例宫颈癌患者中，原位杂交法检测ＨＰＶＤＮＡ有２８例阳性（４３．１％）；Ｌ１ＰＣＲ和Ｅ６ＰＣＲ检测ＨＰＶＤＮＡ阳性分别为１３例（２２．４％）和４５例（７７．６％）。结论：对宫颈癌标本中的ＨＰＶＤＮＡ检测，Ｅ６特异性引物ＰＣＲ敏感性最高，Ｌ１ＰＣＲ及原位杂交相对较低。新疆维吾尔族妇女宫颈癌发病与ＨＰＶ感染有密切关系。
Objective: To study the relationship between cervical cancer and human papillomavirus (HPV) infection among Xinjiang Uygur women with high prevalence of cervical cancer in our country. Methods: HPV16 / 11, 16/18 and 31/33/35 DNA were detected by in situ hybridization in 65 cases of uterine cervix cancer in Xinjiang Uygur women. Polymerase chain reaction (PCR) and E6 specific primers Among them, 58 HPV6, 16 and 18 DNA were detected by PCR. Results: In 65 cases of cervical cancer, HPVDNA was detected in 28 cases (43.1%) by in situ hybridization. The positive rates of HPV DNA in L1PCR and E6PCR were 13 cases (22.4%) and 45 cases (77.6% ). Conclusion: PCR detection of HPV DNA in cervical cancer specimens has the highest PCR sensitivity to E6-specific primers and relatively low L1PCR and in situ hybridization. Uyghur women in Xinjiang have a close relationship with the incidence of cervical cancer and HPV infection.