米诺环素对大鼠脑出血损伤的保护作用及对Bcl-2和MMP-9表达的影响

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目的观察B细胞淋巴瘤/白血病-2(Bcl-2)和基质金属蛋白酶-9(MMP-9)在大鼠脑出血后脑损伤机制中的作用及米诺环素(minocycline)对脑损伤的保护作用。方法 Wistar大鼠随机分为3组:假手术组(Sham)、模型组(Model)及米诺环素组(Minocycline),每组又按时间点不同分为6,12 h,1,2,3,5,7 d亚组。根据大鼠脑立体定位术,制备大鼠脑内囊出血模型。采用神经系统功能评分和体感诱发电位对脑出血模型进行评价。并于不同时间点开颅取脑组织对针眼前脑组织进行含水量的测定,针眼后脑组织制成病理切片,苏木精-伊红染色法用于脑组织形态学的观察,免疫组化染色用于检测出血周围脑组织Bcl-2和MMP-9蛋白的表达情况。结果大鼠内囊出血模型制备成功。免疫组化结果表明,与假手术组相比,模型组各时间点出血周围脑组织的Bcl-2和MMP-9蛋白表达增加(P<0.001),脑组织含水量增加(P<0.001);与模型组相比,米诺环素组各时间点出血周围脑组织Bcl-2表达明显增加,MMP-9表达明显下降,脑组织含水量明显下降。结论米诺环素对脑出血后脑损伤有保护作用,可能与Bcl-2表达增加以及MMP-9表达减少有关。 Objective To investigate the role of Bcl-2 and MMP-9 in brain injury after intracerebral hemorrhage in rats and the protective effect of minocycline on brain injury effect. Methods Wistar rats were randomly divided into 3 groups: sham group, model group and minocycline group. Each group was divided into 6, 12, 3,5,7 d subgroup. According to the rat brain stereotaxic surgery, intracerebral hemorrhage model of rat was made. The cerebral hemorrhage model was evaluated using neurological function scores and somatosensory evoked potentials. At different time points, the cranial brain tissue was taken to measure the water content in the anterior segment of the eyeball. The brain tissue of the eye was made into pathological sections. The hematoxylin-eosin staining was used to observe the morphological changes of the brain tissue. Immunohistochemical staining Used to detect the expression of Bcl-2 and MMP-9 in brain tissue around the hemorrhage. Results The rat model of internal capsule hemorrhage was successfully established. The results of immunohistochemistry showed that compared with the sham group, the expression of Bcl-2 and MMP-9 increased (P <0.001) and the brain water content increased (P <0.001) in the model group at each time point. Compared with the model group, Bcl-2 expression in the brain tissue around the hemorrhage in the minocycline group was significantly increased, the expression of MMP-9 was significantly decreased, and the water content in the brain tissue was significantly decreased. Conclusion Minocycline has a protective effect on brain injury after intracerebral hemorrhage, which may be related to the increase of Bcl-2 expression and the decrease of MMP-9 expression.
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