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目的 观察加入外源性SOD对辐射诱发人血淋巴细胞染色单体型畸变和染色体型畸变的影响。方法 (1)人血 37℃培养 70h后加入不同浓度SOD ,接受 1Gy60 Co -γ射线照射 ,再培养 3h ,按常规方法制片 ,每组镜检 30 0个细胞 ,观察SOD对辐射诱发染色单体型畸变的影响 ;(2 )人血先加入不同浓度SOD ,再按上述条件照射 1Gy ,37℃培养 5 4h ,按常规方法制片 ,每组镜检 30 0个细胞 ,观察SOD对辐射诱发染色体型畸变的影响。 结果 (1)各个浓度SOD(5× 10 -3 ～ 5× 10 -1mg/ml )对辐射诱发的染色单体型互换和断裂均有非常明显的降低作用 ;(2 )只有中浓度SOD对染色体性畸变包括染色体互换与断片 ,以及高浓度SOD对染色体互换才产生降低作用。结论 在上述实验条件下 ,不同浓度SOD对辐射诱发的染色单体型畸变和染色体型畸变均有不同程度降低作用。
Objective To investigate the effects of exogenous SOD on the haplotypic and chromosomal aberrations induced by radiation in human lymphocytes. Methods (1) After human blood was cultured at 37 ℃ for 70h, different concentrations of SOD were added and irradiated with 1Gy60Co-γ ray. The cells were cultured for 3h. The cells were prepared according to routine methods. (2) human blood first added different concentrations of SOD, according to the above conditions irradiation 1Gy, 37 ℃ cultured 5 4h, according to the conventional method of preparation, each group microscopic examination of 30 0 cells were observed SOD radiation-induced Chromosome aberrations. Results (1) The concentration of SOD (5 × 10 -3 ~ 5 × 10 -1 mg / ml) significantly reduced the radiation-induced haplotype exchange and cleavage. (2) Chromosomal aberrations include chromosomal interchanging and fragmentation, as well as the high concentration of SOD to chromosomal exchange was reduced. Conclusion Under the above experimental conditions, different concentrations of SOD have different degrees of reduction on radiation induced haplotypic and chromosomal aberrations.