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【目的】荔枝蒂蛀虫Conopomorpha sinensis Bradley是专一性危害我国华南地区荔枝和龙眼的重要害虫,隐蔽性强,防治困难,基因组信息缺乏。本研究的目的是获得荔枝蒂蛀虫的基因数据,寻求有效控制害虫的分子靶标。【方法】采用新一代高通量测序技术Illumina Hi SeqTM4000对荔枝蒂蛀虫进行转录组测序和生物信息学分析。【结果】经序列拼接获得68 996条unigenes。进一步利用七大公共数据库进行同源比对,注释了22 348 unigenes。注释到Nr数据库的unigenes数量最多,达27.01%,其中Nr注释的荔枝蒂蛀虫unigenes中与小菜蛾Plutella xylostella unigenes同源性最高,达34.1%。将unigenes与GO数据库比对发现,15 585条unigenes根据其功能大致可分为3类47亚类。KEGG pathways分析表明,7 272条unigenes定位为267个代谢通路。基因注释进一步筛选鉴定获得100个荔枝蒂蛀虫嗅觉相关基因;与鳞翅目相关气味结合蛋白基因联合分析发现,与荔枝蒂蛀虫气味结合蛋白基因直系同源的有18组,部分基因形成独立一簇。【结论】本研究首次获得了荔枝蒂蛀虫的转录组数据,研究结果为生物控制荔枝蒂蛀虫提供了重要的基础数据和候选分子靶标。荔枝蒂蛀虫独有的气味结合蛋白基因可能与其生境中特有的化学物质相关。
【Objective】 Conopomorpha sinensis Bradley is an important pest that harms litchi and longan in South China. It has the characteristics of strong concealment, prevention and control, and lack of genome information. The purpose of this study was to obtain the genetic data of Lychee ’s borers and to find the molecular targets for controlling pests effectively. 【Method】 Transcriptome sequencing and bioinformatic analysis of litchi moth were carried out using Illumina Hi SeqTM 4000, a new generation high-throughput sequencing technique. 【Result】 68 996 unigenes were obtained by sequential splicing. Further use of seven public databases for homology alignment, annotated 22 348 unigenes. The number of unigenes annotated to the Nr database was the highest (27.01%). Among them, the Nr annotated litchi borer unigenes had the highest homology with Plutella xylostella unigenes (34.1%). Comparing unigenes with the GO database, 15 585 unigenes can be broadly categorized into three categories and 47 subtypes based on their function. KEGG pathways analysis showed that 7 272 unigenes were mapped to 267 metabolic pathways. The results showed that there were 18 orthologs of odorant binding protein genes in Litchi chinensis and some genes formed an independent cluster . 【Conclusion】 This study was the first to obtain the transcriptome data of Lychee dorsalis, and the results provided important basic data and candidate molecular targets for biological control of Lychee dorsalis. The unique odorant binding protein gene of litchi borer may be related to the unique chemicals in its habitat.