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The flexural behaviors of continuous fully and partially prestressed steel fiber reinforced high strength concrete beams are studied by experiment and nonlinear finite element analysis. Three levels of partial prestress ratio (PPR) are considered, and three pairs of two-span continuous beams with box sections varying in size are designed. The major parameters involved in the study include the PPR and the fiber location. It is concluded that the prestressed high strength concrete beam exhibits satisfactory ductility; the influences of steel fiber on the crack behaviors for partially prestressed beams are not as obvious as those for fully prestressed ones; steel fibers can improve the structural stiffness after cracking for fully prestressed high strength concrete beams; the moment redistribution from mid-span to intermediate support in the first stage should be mainly considered in practical design.