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为探讨抗丙型肝炎病毒（ＨＣＶ）ＩｇＧ型抗体及其亚群在ＨＣＶ感染过程中的作用，采用酶联免疫吸附法对６６例输血后ＨＣＶ感染患儿的９２份急、慢性期血清和３２份α干扰素（αＩＦＮ）治疗后血清进行抗Ｃ２２、抗Ｃ３３Ｃ和抗ＮＳ５及其亚群的检测。结果表明，急性期抗Ｃ２２和抗Ｃ３３Ｃ检出率高，分别为９８．４３％和１００．００％；而抗ＮＳ５在急性期检出率仅为５３．１２％，明显低于慢性期（９１．６７％）和同期抗Ｃ２２和抗Ｃ３３Ｃ检出率（分别为９６．８８％和１００．００％）。三种抗体ＩｇＧ亚群在急性期的检出率和抗体水平均显著低于慢性期。抗Ｃ２２ＩｇＧ１为该抗体的优势亚群；而抗Ｃ３３Ｃ无明显优势亚群；抗ＮＳ５ＩｇＧ１及ＩｇＧ３至慢性期才显示其优势。６６例患儿中，１３例接受αＩＦＮ治疗，其中５例复发患儿在ＨＣＶＲＮＡ再次出现时，抗Ｃ２２ＩｇＧ１呈现有意义的下降，而抗Ｃ２２总水平并无明显变化，而接受αＩＦＮ治疗４例有效和另４例无效患儿无类似变化。提示抗Ｃ２２和抗Ｃ３３Ｃ是诊断急性ＨＣＶ感染的敏感指标；抗Ｃ２２ＩｇＧ１可能有助于评价αＩＦＮ治疗ＨＣＶ感染的疗效。
In order to investigate the role of anti-HCV IgG and its subgroups in HCV infection, enzyme linked immunosorbent assay (ELISA) was used to detect the serum levels of 92 acute and chronic sera from 66 post-transfusion HCV infected patients and 32 Serum samples were tested for anti-C22, anti-C33C, anti-NS5 and their sub-populations after IFN-alpha treatment. The results showed that the detection rates of anti-C22 and anti-C33C were 98.43% and 100.00% in acute phase, while the detection rate of anti-NS5 in acute phase was only 53.12%, which was significantly lower than that in chronic phase .67%) and the same period anti-C22 and anti-C33C detection rate (96.88% and 100.00% respectively). The detection rates and antibody levels of the three subgroups of IgG in the acute phase were significantly lower than those in the chronic phase. Anti-C22IgG1 is the dominant sub-group of this antibody; while anti-C33C had no obvious superior subgroup; anti-NS5IgG1 and IgG3 showed their advantages only in chronic phase. Of the 66 children, 13 were treated with αIFN, and 5 of the 5 relapsed children showed a significant decrease in anti-C22 IgG1 at the time of recurrence of HCVRNA, whereas there was no significant change in the overall level of anti-C22, whereas 4 patients treated with αIFN were effective The other 4 cases of invalid children with no similar changes. It is suggested that anti-C22 and anti-C33C are sensitive indicators for the diagnosis of acute HCV infection. Anti-C22 IgG1 may be helpful to evaluate the therapeutic effect of aIFN on HCV infection.