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目的探讨不同氧浓度对卵裂胚复苏妊娠结局的影响。方法选取2014年1月-2016年6月该院818个卵裂胚周期进行胚胎培养研究,其中低氧浓度组231个卵裂胚,低氧浓度采用(5%O2,6%CO2,89%N2)过夜培养。常规氧浓度组587个周期,采用(6%CO2,94%空气)过夜培养,分析对比两组临床妊娠率、种植率、多胎率和流产率情况。结果低氧组子宫内膜准备方案为自然周期的占60.82%,显著高于常规氧浓度组的48.05%(P<0.05);低氧组激素替代周期占19.76%,明显低于常规氧浓度组的38.10%(P<0.05);两组胚胎复苏率(95.90%vs.96.41%)和移植优胚率(74.98%vs.72.67%)差异无统计学意义(P<0.05),低氧组的胚胎种植率和临床妊娠率明显高于常规氧浓度组(P<0.05),多胎率和流产率低于常规氧浓度组,但差异无统计学意义(P>0.05)。结论低氧浓度对卵裂胚复苏妊娠结局具有更好的作用。
Objective To investigate the effect of different oxygen concentrations on the pregnancy outcome of cleavage embryo resuscitation. Methods From January 2014 to June 2016, 818 cleavage embryos of this hospital were selected for embryo culture. 231 hypocotyl embryos in low oxygen concentration group were selected and hypoxia concentrations were determined using 5% O2, 6% CO2 and 89% N2) overnight. Conventional oxygen concentration group 587 cycles, using (6% CO2, 94% air) overnight culture, analysis and comparison of two groups of clinical pregnancy rate, implantation rate, multiple pregnancy rate and abortion rate. Results The endometrial preparation program in hypoxia group was 60.82% of the natural period, significantly higher than 48.05% (P <0.05) in the conventional oxygen concentration group. The hormone replacement period in the hypoxia group was 19.76%, which was significantly lower than that in the conventional oxygen concentration group (P <0.05). There was no significant difference between two groups (95.90% vs.96.41%) and embryo transfer rate (74.98% vs.72.67% Embryo implantation rate and clinical pregnancy rate were significantly higher than the conventional oxygen concentration group (P <0.05), multiple birth rate and abortion rate was lower than the conventional oxygen concentration group, but the difference was not statistically significant (P> 0.05). Conclusions Hypoxia has a better effect on the pregnancy outcome of cleavage embryo resuscitation.