C_3和C_4作物杂交,因其亲缘关系较远,即使在室内人工控制的条件下进行杂交,其结实率也甚低。而且所获得的杂种种子多数为无胚乳或胚乳极少的干瘪畸型种子或种胚。这类种子或种胚直播于田土内,一般是不能发芽和成苗的。因之,采用适宜的培养基和培养条件,是获得杂种幼苗的关键。 C_3 and C_4 crop hybridization, because of their genetic relationship, even under the conditions of artificial control indoor hybridization, its seed setting rate is very low. Moreover, the majority of the hybrid seeds obtained were avascularly deformed seeds or embryos with few endosperm or endosperm. Such seeds or seed embryo live in the soil, the general can not germinate and seedlings. Therefore, the use of appropriate media and culture conditions, is the key to obtaining hybrid seedlings.