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目的分析结核感染T细胞斑点试验应用于快速诊断HIV/AIDS合并结核病的临床应用价值。方法选取2014年1月~2016年1月我院收治的结核分枝杆菌与HIV病毒双重感染患者50例及单纯结核病患者50例,应用T-SPOT.TB对患者的血液标本进行结核分枝杆菌特异性检测,同时对所有检测患者做PPD皮试,结核抗体检测,抗酸杆菌涂片试验进行比较。结果在艾滋合并结核病的患者中,T-SPOT.TB与PPD皮试、结核抗体检测、抗酸杆菌涂片检测的阳性率分别为67.2%,22.1%,20%,18%,T-SPOT.TB检测的阳性率显著高于传统检测方法,差异有统计学意义(P<0.05);单纯结核病患者中,T-SPOT.TB与PPD皮试、结核抗体检测、抗酸杆菌涂片检测的阳性率分别为89%,69%,70%,24%,T-SPOT.TB检测的阳性率显著高于传统检测方法,差异有统计学意义(P<0.05)。结论 T-SPOT.TB检测的敏感度显著高于传统检测方法,适用于我国艾滋合并结核的快速诊断。
Objective To analyze the clinical value of T-cell spot test of tuberculosis infection in the rapid diagnosis of HIV / AIDS complicated with tuberculosis. Methods Fifty patients with dual infection of Mycobacterium tuberculosis and HIV and 50 patients with simple tuberculosis admitted to our hospital from January 2014 to January 2016 were enrolled. T-SPOT.TB was used to detect Mycobacterium tuberculosis Specificity tests were performed at the same time. PPD skin tests, tuberculosis antibody tests and acid-fast bacilli smear tests were performed on all tested patients. Results The positive rates of T-SPOT.TB and PPD skin test, tuberculosis antibody test and acid-fast bacilli smear were 67.2%, 22.1%, 20%, 18% and T-SPOT in patients with AIDS complicated with tuberculosis. TB test was significantly higher than the traditional detection methods, the difference was statistically significant (P <0.05); simple tuberculosis patients, T-SPOT.TB and PPD skin test, tuberculosis antibody test, acid-fast bacilli smear positive The positive rate of T-SPOT.TB was 89%, 69%, 70% and 24% respectively, which was significantly higher than that of the traditional methods (P <0.05). Conclusion The sensitivity of T-SPOT.TB detection is significantly higher than that of traditional detection methods, which is suitable for the rapid diagnosis of HIV-associated tuberculosis in China.