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目的探讨胰岛素样生长因子（ＩＧＦ１）对人牙周膜细胞（ＰＤＬｃｅｌｓ）胶原合成的调节及ＩＧＦ１作用的细胞内分子机理，特别是多聚腺嘌呤二磷酸核糖（ＰＡＤＰＲ）反应在ＩＧＦ１调节胶原合成中的意义。方法用体外培养的人牙周膜细胞（８～１０代），观察不同浓度的ＩＧＦ１（１０～１００μｇ／Ｌ）作用１６小时后牙周膜细胞胶原合成以及细胞内乳酸代谢、ＰＡＤＰＲ合成和ＰＡＤＰＲ合成酶活性的关系。结果ＩＧＦ１能明显刺激牙周膜细胞胶原合成，同时伴随乳酸产生的增加和ＰＡＤＰＲ合成酶活性下降、ＰＡＲＰＲ合成减少。结论ＩＧＦ１可能通过影响ＰＡＤＰＲ合成而调节牙周膜细胞胶原合成。
Objective To investigate the effects of insulin-like growth factor (IGF1) on the collagen synthesis of human periodontal ligament cells (PDLcels) and the intracellular molecular mechanism of IGF1. In particular, poly (ADA) ribose ribose (PADPR) Meaning. Methods Human periodontal ligament cells (8-10 passages) were cultured in vitro and the collagen synthesis, intracellular lactate metabolism, PADPR synthesis and PADPR synthesis of periodontal ligament cells were observed after 16 hours exposure to various concentrations of IGF1 (10-100 μg / L) Relationship between enzyme activity. Results IGF1 stimulated the collagen synthesis of periodontal ligament cells, accompanied by the increase of lactate production and the decrease of PADPR synthase activity, and decreased the synthesis of PARPR. Conclusion IGF1 may regulate collagen synthesis in periodontal ligament cells by affecting the synthesis of PADPR.