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电镜酶细胞化学方法是在光镜酶组织化学基础上发展起来的。酶的检出法有金属沉淀法和色素法。金属沉淀法常以铅盐、铜盐、钡盐等金属作为捕捉剂。其中铅盐是比较理想的捕捉剂。铅捕捉剂广泛用于检出磷酸酶,酯酶和转移;在酸性、碱性、中性等PH范围内均可使用;而且反应一次完成。色素法有偶氮色素法、二氨基联苯胺
Electron microscopy Enzyme cytochemistry is developed on the basis of light microscopy and histochemistry. Enzymes detection method with metal precipitation method and the pigment method. Metal precipitation often lead, copper, barium salts and other metals as a capture agent. Lead salt which is the ideal capture agent. Lead scavengers are widely used to detect phosphatase, esterase and transfer; in acid, alkaline, neutral pH range can be used; and the reaction is completed. Pigment method azo dye method, diaminobenzidine