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目的 考察重组人乳铁蛋白自身及其在奶粉中的抗氧化活性.方法 通过二苯代苦味肼基(DPPH)自由基抑制率、羟基自由基抑制率、超氧阴离子自由基抑制率、还原能力和抑制大鼠肝脏体外脂质过氧化等试验评价重组人乳铁蛋白和牛乳铁蛋白的体外抗氧化性能.结果 重组人乳铁蛋白和牛乳铁蛋白的浓度与DPPH自由基抑制率、抑制羟基自由基能力、抑制超氧阴离子自由基能力和还原能力之间呈明显剂量-效应关系,且差异有统计学意义(P＜0.05);样本和浓度之间没有交互作用,重组人乳铁蛋白和牛乳铁蛋白在不同浓度下所得值的趋势相同.重组人乳铁蛋白和牛乳铁蛋白在对大鼠肝组织匀浆抗氧化性影响差异有统计学意义(P＜0.05),不同浓度乳铁蛋白之间差异有统计学意义(P＜0.05).结论 重组人乳铁蛋白和牛乳铁蛋白具有一定的抗氧化活性,可以添加到保健品或者化妆品中作为天然抗氧化剂.“,”Objective To investigate the antioxidant activity of recombinant human lactoferrin (rhLF).Methods The 1,1-diphenyl-2-picryl hydrazyl free radical (DPPH) inhibiting rate,hydroxyl free radical inhibiting rate,superoxide anion radical inhibiting rate,reducing capacity and inhibiting of lipid peroxidation in rat liver were tested to evaluate the antioxidant activity of rhLF and bovine lactoferrin (bLF) in vitro.Results The concentration of rhLF and bLF were correlated with the inhibiting rate of 1,1-diphenyl-2-picryl hydrazyl free radical,hydroxyl free radical,superoxide anion radical and reducing capacity.There was significantly dose-response relationship and the difference had statistical significance (P ＜ 0.05).There was no interaction between sample and concentration,the trend of the value was the same in different concentrations of rhLF and bLF.Antioxidant effect of rhLF and bLF had significant difference in rat liver homogenate,different concentrations of rhLF and bLF had significant difference (P ＜ 0.05).Conclusion Bovine lactoferrin and rhLF have antioxidant activity,and could be added to health food or cosmetics as a natural antioxidant.