The motor and trailer cars of a high-speed train were modeled as a multi-rigid body system with two suspensions. According to structural characteristic of a slab track, a new spatial vibration model of track segment element of the slab track was put forward. The spatial vibration equation set of the high-speed train and slab track system was then established on the basis of the principle of total potential energy with stationary value in elastic system dynamics and the rule of “set-in-right-position” for formulating system matrices. The equation set was solved by the Wilson-θ direct integration method. The contents mentioned above constitute the analysis theory of spatial vibration of high-speed train and slab track system. The theory was then verified by the high-speed running experiment carried out on the slab track in the Qinghuangdao-Shenyang passenger transport line. The results show that the calculated results agree well with the measured results, such as the calculated lateral and vertical rail displacements are 0.82 mm and 0.9 mm and the measured ones 0.75 mm and 0.93 mm, respectively; the calculated lateral and vertical wheel-rail forces are 8.9 kN and 102.3 kN and the measured ones 8.6 kN and 80.2 kN, respectively. The interpolation method, that is, the lateral finite strip and slab segment element, for slab deformation proposed is of simplification and applicability compared with the traditional plate element method. All of these demonstrate the reliability of the theory proposed. The motor and trailer cars of a high-speed train were modeled as a multi-rigid body system with two suspensions. According to structural characteristic of a slab track, a new spatial vibration model of track segment element of the slab track was put forward. The spatial vibration equation set of the high-speed train and slab track system was then established on the basis of the principle of total potential energy with stationary value in elastic system dynamics and the rule of “set-in-right-position ” The equation set was solved by the Wilson-θ direct integration method. The contents mentioned above constitute the analysis theory of spatial vibration of high-speed train and slab track system. The theory was then verified by the high-speed running experiment carried out on the slab track in the Qinghuangdao-Shenyang passenger transport line. The results show that the calculated results agree well with the measured results, such as the calculated latera l and vertical rail displacements are 0.82 mm and 0.9 mm and the measured ones are 0.75 mm and 0.93 mm, respectively; the calculated lateral and vertical wheel-rail forces are 8.9 kN and 102.3 kN and the measured ones 8.6 kN and 80.2 kN, respectively. The interpolation method, that is, the lateral finite strip and slab segment element, for slab deformation proposed is simplification and applicability compared with the traditional plate element method. All of these demonstrated the reliability of the theory proposed.