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草菇菌种多采用继代培养常温保藏法.为减少草菇菌种转接次数保持菌种活力,近年来我们用稻草作基质试管蜡封保藏,效果较好,现将方法介绍如下:取新鲜晒干的稻草去掉叶梢,将草茎剪成约1cm长小段,用清水浸泡半小时,使草含水60%左右,加10%葡萄糖拌匀.选用18×180mm的试管,先在试管底部放一层厚约1.5～2cm、含水60%的脱脂棉,然后将草料装入,松紧适度,装至试管长度2/3处为宜.清洁试管,塞好棉塞置1.5kg/cm~2压力下灭菌40分钟,
In order to reduce the number of transfer of mushroom strains to maintain the viability of bacteria, in recent years we use straw as a matrix tube wax seal, the effect is better, the method described below: Fresh straw dried to remove the tip, the grass stems cut into small pieces about 1cm, soaked in water for half an hour, so that the grass about 60% water, add 10% glucose and mix well.Use 18 × 180mm tube, first in the bottom of the tube Put a layer of about 1.5 ~ 2cm, 60% moisture absorbent cotton, and then the forage into the tight, moderately loaded to the tube length 2/3 is appropriate. Clean the test tube, plug the plug set 1.5kg / cm ~ 2 pressure 40 minutes under sterilization,