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目的：通过对细胞外间质 ECM 成分进行检测，探讨间质重构与子宫颈鳞状细胞癌侵袭性生长及播散的关系。方法：应用双重免疫荧光染色技术和激光共聚焦显微镜观察，对正常人及不同分化程度的子宫颈鳞状细胞癌患者的子宫颈石蜡包埋组织切片纤维结合素 FN、层粘连蛋白 LN 及Ⅰ、Ⅲ、Ⅳ型胶原蛋白的表达进行检测。结果：正常子宫颈组织中，ECM内可见结构致密的Ⅰ、Ⅲ型胶原和细条索状的FN蛋白表达，Ⅳ型胶原和LN蛋白主要呈线状分布于基底膜内。子宫颈鳞状细胞癌组织中，Ⅰ、Ⅲ和Ⅳ型胶原降解，尤以后者为著，表现为Ⅰ、Ⅲ型胶原结构稀疏，排列紊乱，基底膜内Ⅳ型胶原的线状荧光减弱、断裂甚至缺失，间质和癌细胞浆内FN和LN蛋白表达增多，胶原的降解和FN、LN表达与癌细胞分化程度负相关。结论：子宫颈鳞状细胞癌，间质胶原降解，基底膜断裂，FN及LN表达增多，ECM重构，其程度与癌细胞分化程度呈负相关。
OBJECTIVE: To investigate the relationship between interstitial remodeling and aggressive growth and dissemination of cervical squamous cell carcinoma (ECM) by detecting extracellular matrix ECM. Methods: Double immunofluorescence staining and confocal laser scanning microscopy were used to detect the expression of fibronectin, fibronectin, laminin, and laminin in cervical squamous cell carcinoma of normal and differentiated cervical squamous cell carcinoma and Ⅰ, Ⅲ, Ⅳ collagen protein expression was detected. Results: In normal cervical tissue, densely organized type I and type III collagens and fine cord-like FN protein expression were observed in ECM. Type IV collagen and LN protein were mainly distributed in the basement membrane. Cervical squamous cell carcinoma, Ⅰ, Ⅲ and Ⅳ type collagen degradation, especially in the latter as the performance of Ⅰ, Ⅲ type collagen structure is sparse, disordered arrangement, basement membrane collagen IV linear fluorescence weakening, fracture Even missing, interstitial and cancer cell plasma FN and LN protein expression increased collagen degradation and FN, LN expression and cancer cell differentiation is negatively correlated. Conclusion: Cervical squamous cell carcinoma, interstitial collagen degradation, basement membrane rupture, FN and LN expression increased, ECM remodeling, the degree of cancer cells and the degree of differentiation was negatively correlated.